Category: Uncategorized

Research use only. The information below concerns laboratory reference materials for in-vitro research and makes no medical, therapeutic, diagnostic, cosmetic or performance claims.

“Research use only” (RUO) describes a material supplied strictly as an in-vitro laboratory reference standard — not for any human or animal use, not for diagnostic use, and not as a medicinal product. This guide explains how VERIPHASE classifies, documents and supplies RUO peptide reference materials in the European Union.

Intended purpose

Under EU rules a product is defined by its intended purpose. An RUO reference material is intended only for in-vitro research; it is not a medicinal product, food supplement, cosmetic or in-vitro diagnostic device, and no therapeutic, diagnostic or performance claims are made about it.

Documentation

Each released lot is characterised by an accredited third-party laboratory and accompanied by a certificate of analysis (COA) covering identity and purity. Intrinsic chemistry — sequence, molecular formula, molecular weight and CAS number — is published per product for assay planning.

Enquiry-only supply during soft launch

Online ordering is not yet open. Researchers can browse the catalogue and request availability and lot documentation through the contact page. Supply is intended for qualified research organisations within the EU.

Research use only. The information below concerns laboratory reference materials for in-vitro research and makes no medical, therapeutic, diagnostic, cosmetic or performance claims.

A certificate of analysis (COA) records what an independent laboratory measured for one specific lot of a reference material. Reading it correctly is the basis of reproducible in-vitro work.

Identity (LC-MS / ESI-MS)

Mass spectrometry compares the observed mass with the theoretical molecular weight, confirming that the molecule in the vial is the one named on the label.

Purity (RP-HPLC)

Reversed-phase HPLC reports the area-percent of the main peak; a high area-percent indicates a low level of chromatographic impurities in the tested sample.

Net peptide content, water and counterion

Net peptide content, water (Karl Fischer) and the counterion or salt form (for example acetate or trifluoroacetate) affect how much actual peptide a stated mass contains — important when calculating stock concentrations.

Lot traceability

Always check that the lot number on the COA matches the vial. A COA is only meaningful for the exact lot it describes.

Research use only. The information below concerns laboratory reference materials for in-vitro research and makes no medical, therapeutic, diagnostic, cosmetic or performance claims.

Peptide reference materials are sold under many overlapping names, aliases and abbreviations. This glossary clarifies the terms a researcher needs to match a catalogue entry to the correct molecule.

Common names and aliases

• BPC-157 — a synthetic pentadecapeptide reference standard.
• TB-500 — commonly the N-acetylated fragment of Thymosin β4; distinct from full-length Thymosin β4.
• CJC-1295 No-DAC — also called Mod GRF (1-29); distinct from DAC-modified CJC-1295.
• Ipamorelin — a pentapeptide reference standard.
• GHK-Cu — the copper(II) complex of glycyl-L-histidyl-L-lysine; Copper Tripeptide-1 is included as an INCI cross-reference name.
• MOTS-c — a 16-amino-acid mitochondrial-derived peptide.

Fragments vs full-length

A fragment is a defined sub-sequence of a larger peptide with its own molecular formula, mass and CAS number. Confusing a fragment with the full-length molecule leads to incorrect mass and concentration calculations.

Salt forms

Reference materials are often supplied as acetate or trifluoroacetate salts. The salt form changes the total mass, so net peptide content must be used when preparing stock solutions.

Research use only. The information below concerns laboratory reference materials for in-vitro research and makes no medical, therapeutic, diagnostic, cosmetic or performance claims.

“TB-500” and “Thymosin β4” are often treated as synonyms, but for reference-material labelling they can denote two different molecules. This note explains the distinction so the correct identity data is used.

The fragment

“TB-500” most precisely denotes the N-acetylated fragment of Thymosin β4 containing the actin-binding motif (residues 17-23), with its own molecular formula, molecular weight and CAS number.

The full-length protein

Full-length Thymosin β4 is a 43-amino-acid peptide with a substantially higher molecular weight and a different CAS number. Some suppliers label full-length material as “TB-500”.

Why it matters for the lab

Because the two forms differ in mass, a concentration calculated for one is incorrect for the other. Always confirm which molecule a COA and its mass-spectrometry identity describe before using the data. VERIPHASE lists the fragment and the full-length form as separate reference materials.

Research use only. The information below concerns laboratory reference materials for in-vitro research and makes no medical, therapeutic, diagnostic, cosmetic or performance claims.

CJC-1295 is available in two distinct reference-material forms. They share a core sequence but differ structurally, which changes their molecular weight and identifiers.

No-DAC (Mod GRF 1-29)

CJC-1295 “without DAC”, also called Modified GRF (1-29), is a 29-residue growth-hormone-releasing-hormone analogue, often listed simply as “Mod GRF 1-29”.

With DAC

The “DAC” form carries a Drug Affinity Complex modification, giving a higher molecular weight and a different CAS number.

Matching the reference standard

For assay design the No-DAC and DAC forms are not interchangeable: their masses and identifiers differ, so the COA must match the intended molecule. VERIPHASE supplies CJC-1295 No-DAC (Mod GRF 1-29) as an in-vitro reference standard.

Research use only. The information below concerns laboratory reference materials for in-vitro research and makes no medical, therapeutic, diagnostic, cosmetic or performance claims.

Preparing an in-vitro stock solution from a lyophilised reference material is straightforward arithmetic. This guide covers the calculations; our laboratory stock-concentration & aliquot calculator performs them automatically.

Stock concentration

Divide the reference-material mass by the volume of solvent added. A 5 mg reference dissolved in 2 mL of solvent gives a stock concentration of 2.5 mg/mL.

Aliquot volume

To calculate an aliquot volume for a target mass, divide the target mass by the stock concentration. At 2.5 mg/mL, a 250 µg aliquot is 0.10 mL (100 µL).

Net peptide content

For salt forms, use the net peptide content from the COA rather than the gross vial mass, so the calculated concentration reflects the actual peptide present.

Handling

Store lyophilised material cold, dry and dark; split prepared stock into aliquots to minimise repeated freeze-thaw events. These calculations are for in-vitro laboratory planning only.