Research use only. The information below concerns laboratory reference materials for in-vitro research and makes no medical, therapeutic, diagnostic, cosmetic or performance claims.
Preparing an in-vitro stock solution from a lyophilised reference material is straightforward arithmetic. This guide covers the calculations; our laboratory stock-concentration & aliquot calculator performs them automatically.
Stock concentration
Divide the reference-material mass by the volume of solvent added. A 5 mg reference dissolved in 2 mL of solvent gives a stock concentration of 2.5 mg/mL.
Aliquot volume
To calculate an aliquot volume for a target mass, divide the target mass by the stock concentration. At 2.5 mg/mL, a 250 µg aliquot is 0.10 mL (100 µL).
Net peptide content
For salt forms, use the net peptide content from the COA rather than the gross vial mass, so the calculated concentration reflects the actual peptide present.
Handling
Store lyophilised material cold, dry and dark; split prepared stock into aliquots to minimise repeated freeze-thaw events. These calculations are for in-vitro laboratory planning only.